Yeast Expression Services

Open Pichia Expression Services

Why Pichia?

Open Pichia is Kemp’s fully open, IP-free Pichia expression platform, offering high-performance recombinant protein expression that is flexible, fast, and built for scale. Designed for maximum flexibility without licensing barriers or proprietary lock-ins, it uses transparent methods, customizable vectors, and modular workflows to let you develop, optimize, and scale your program on your own terms. Kemp Protein offers end-to-end support for reliable, cost-effective expression in Pichia pastoris (Komagataella phaffii). Whether you need feasibility testing, Kg-scale production, or full strain development with tech transfer, our modular service suite is designed to fit seamlessly into your workflow at any stage. Pichia pastoris offers an ideal balance of eukaryotic protein processing, robust fermentation, and low production cost. With strong secretion pathways, high cell density fermentation, and customizable induction systems (including methanol-free options), Pichia is a top choice for enzymes, biologics, diagnostics, and industrial proteins.

Our Services

Feasibility & Expression Screening
Rapid readouts on Pichia expression. Codon optimization, vector/promoter and secretion-signal screening, and small-scale expression runs—ending with a clear Go/No-Go.
Timeline: 4–6 weeks. Deliverables: Data package plus pellets/lysate/supernatant.

Strain Construction & Engineering
Custom, sequence-verified strains built for performance: stable integration, multi-copy clones, promoter/pathway tuning, and glycoengineering.
Deliverables: RCB, expression data, vector maps.

Fermentation & Process Development
High-density fed-batch fermentation and optimization of DO, feed, temp, and induction. Methanol or methanol-free.
Scales: 1 L to 50 L pilot with optional transfer to manufacturing partners.

Purification & Analytics
Purity and performance delivered. Affinity, IEX, SEC, and custom chromatography; polishing and buffer formulation; SDS-PAGE, Western, HPLC, SEC, and activity assays.
Deliverables: mg–Kg purified protein with full analytics.

Scale-Up & Tech Transfer
Seamless transition into production with process transfer packages, GMP-aligned documentation, manufacturing coordination, and team training.

What Makes Us Different

Open & Flexible – Your vectors or ours, full transparency, modular workflows.
Fast Turnaround – Built for quick decisions and early yield insight.
High Success Rate – Deep Pichia expertise and optimized systems.
Scale-Ready – Processes designed for downstream success.

Ideal Applications

Enzymes • Diagnostic proteins • Antibody fragments & scaffolds • Vaccine antigens • Complex eukaryotic proteins not suited for E. coli

How to Get Started

Share your sequence, receive a tailored workplan and quote, and kick off—most feasibility projects start within 5–7 business days.