Monoclonal Antibody Production Service

Kemp Proteins has a long history of producing monoclonal antibodies—both IgG and IgM—from a variety of hybridoma cell lines, including mouse, rat, guinea pig, rabbit, and human. We deliver milligram to gram quantities of low-endotoxin and purified antibody to customer specifications, supported by quality documentation (including a Certificate of Analysis).

Kemp Proteins is flexible in production scale (mg to gm quantities) and, once we have assessed the hybridoma, we guarantee production output. When possible, we transfer hybridoma production into serum-free media to facilitate purification and low endotoxin levels. We offer several production platforms appropriate to client needs and quantity, including single-use bioreactors (including Wave), spinner flasks, stirred tank, membrane flasks, and roller bottles.

Cell Line Transfer Form
If you are interested in Kemp’s monoclonal antibody production service, please fill in and submit the Cell Line Transfer form, and a technical representative will contact you within 1 business day to discuss your project.

Monoclonal Antibody Production Details:

Hybridoma Source:

We accept hybridoma cell lines directly from clients or from third-party sources, or we can develop hybridoma cell lines for the project. If the hybridoma cell line is sourced from the client or from a third party, we require:

Mycoplasma-negative confirmation
Species of origin
Known productivity levels
Known required media components
Antibody isotype
Two vials of frozen cells

Antibody Purification:

Our purification group provides both fully automated (BioRad NGC & GE AKTAPilot) and manual purification techniques performed in an ISO 7 / Class 10,000 environment. Our team is experienced with most standard purification media, including Protein A, Protein G, Protein A/G, and LigaTrap. Final buffer requirements are specified by the client.

Quality Control and Quality Assurance:

For standard hybridoma productions, we routinely perform:

Protein concentration / total antibody
Antibody isotype
pH/ buffer type
Purity
Aggregation
Endotoxin levels

In addition, we consider other quality control assay methods requested by clients—we have qualified various client-driven QC methods and have provided the data as part of the release criteria.

Final Product:

We store final product in a cold room facility with generator back-up until it is shipped. We package and ship (or drop-ship) final product according to client specifications.

Value-Added Processes:
Kemp Proteins offers the following value-added services:

Workflow & Timeline: Antibody Production