Baculovirus Expression Services

Kemp Proteins’ expertise in the baculovirus expression system (BEVS) is well known in the industry, and our team has completed thousands of baculovirus protein expression projects. Over nearly three decades, we have developed robust protocols for the creation of baculovirus constructs, the characterization of expression conditions, and scaleup of protein expression and subsequent purification.

In addition to generating standard baculovirus high-titer stocks, we routinely produce baculovirus-infected insect cells (BIIC) that are cryopreserved as cell banks for use in various expression protocols. We can use three cell lines for production: Sf9, Sf21, and T.ni. We also offer virus plaque purification services, master virus banks, and working virus bank production.

Kemp Proteins has also developed a proprietary PEI-mediated transient transfection protocol using Sf9 and S2 cells for protein expression in shake flasks and stirred tank bioreactors. One advantage of this approach is the ability to rapidly screen variant constructs prior to production. We have been very successful in using this technology to produce VLPs in Sf9 cells at the 2-liter bioreactor scale.

Using these approaches, we have delivered a range of customer-defined proteins, including many difficult-to-express, novel proteins (never previously expressed) that have required our scientists to be innovative in their approach. This breadth of experience makes our team the choice in the industry for expressing your protein in insect cells. Few other companies have the depth of experience represented by the Kemp Proteins team.

We optimize protein expression using titration experiments to determine the optimal virus-to-cell infection ratio and time of harvest from our multi-liter culture in serum-free media formulations.

Cell lysate production, supernatant concentration, and protein purification services are also available.

Use our quick quote form to request a quote.

Not sure which cell line to use? Get in touch.