High Throughput Services

High Throughput Mini & Midi Scale Expression & Purification

High throughput (HTP) gene to antibody production offers Kemp Proteins’ clients a rapid solution to quickly express a large number of antibodies on a small scale. The service utilizes our expertise in high-throughput (HTP) gene synthesis, vector construction, and optimized transient antibody expression technology (mammalian, insect, bacteria) to manufacture high-quality recombinant antibodies with high purity and low endotoxicity.

Kemp Proteins has streamlined our purification process using Protein Maker, Hamilton IMCS tips, and years of experience delivering what our clients need in the timelines that meet their schedules. Kemp Proteins has chromatography systems (Protein Maker and Hamilton IMCS tips) that simultaneously run up to 24-96 columns for:

96-well, 24-well deep plate, 8ml, 40ml, 100ml, 250ml, or 1 L of concentrated supernatants

Clients are looking for:

Fast turnaround: 30 days to get high quality material
Cost efficient pricing
Fully customized expression & purification to meet all requirements
Purified Antibodies in Plates or Individually Vialed.

Midi-Scale Automated Purification of Multispecific AB Fusions

Start your scientific discussion today: request a quote.

High Throughput Mini Mid & Large Expression & Purification Service

Service	Specifications	Timeline
Gene synthesis and codon optimization	Client can provide sequence or client can provide plasmid	7 days
Vector Construction	Expression vector construction Plasmid sequencing Mini or Midi or Large scale plasmid production	7 Days
Expression & Purification	Transient transfection of HEK293 or CHO cells in High Density HD expression Protein A Capture Two Main Bi-Specific Purification Methods: Protein A Capture, in-vitro redox recombination or Protein A Capture, CH1 purification, SEC polish Kemp Proteins purification modalities are flexible and we will adjust our workflow to optimize your success.	7 Days
QC analysis	Analysis by SDS-PAGE & A280 Purity analysis by HPLC-SEC and endotoxin level analysis (optional) Mass Spec – Protein ID or Intact Mass Spec (optional)	3-5 days depending on QC requirements

Deliverable: Purified Antibody & Certificate of Analysis

In this high throughput service, please note that:

Client needs to provide the antibody sequence or plasmid.
A standard service includes: antibody concentration detected by A280 and purity evaluated by SDS-PAGE.
Other services available: SEC-HPLC, endotoxin level test, Protein ID, Intact Mass Spec.

Start your scientific discussion today: request a quote.

What is a Bispecific?

Bispecific antibodies can recognize and bind to two different antigens separately. This allows it to connect immune cells, viral molecules etc. to tumor cells thereby enhancing the killing effect.
Bispecific Classes – Those Bearing an Fc and those without an Fc:

Fc Bearing (IgG like structure)
BSAB
Bispecific (mab)2
Minibody
Quadroma
Multi-specific antibodies

Non-Fc (non-IgG like structures)
bsDb
scBsDb
BiTE
Bispecific Antibody Fragments

Recombinant Bispecific Antibody Approaches

Knobs-Into-Holes (KiH)
Ig-scFv fusion
Controlled Fab-Arm Exchange (cFAE)
Charge-pairs (CP)
CrossMAB

Ask a Scientist about the best approach for your program.

All work is performed under ISO 9001:2015 and ISO 13485:2016 umbrella of quality providing our clients peace of mind that materials, data, and service are performed to the highest level of quality.

Use our quick quote form to request a quote.

Kemp Protein’s Specialty Purification Process

Client communication: We consider this vital to maximizing the potential for a successful project outcome. Our initial discussions are information gathering against a set of questions. The information gained enables us to generate a statement of work, a “project design”, that is shared with you. Together we refine the plan until you are in agreement that we have captured the required specifications and deliverables for the project together with and nuances that may be particular to your project. We will supply regular updates through conference calls or information download through electronic means such as “Box”.

Project Commencement: The project begins when we agree on the design specifications and you provide us any client specific deliverables. Each project is different but runs to a general set approach.

Project Approach: Our approach to specialty purification development is dependent on the project being undertaken. However, they all have certain commonalities. Using a recent project as an example we have outlined the steps for this project – other projects and resins have different steps. We have found this an efficient and effective process to achieve the desired outcome of our clients.