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Gene to Protein Services

Kemp Proteins has more than 20 years of experience providing complete Gene to Protein Services for the life science and research industry. Our team of experienced protein scientists is focused on the timely delivery of high-quality gene-to-protein services, providing the protein you require while also optimizing productivity and mitigating risk in your discovery, development, and manufacturing programs. Using your sequence as the starting point, our team will discuss the requirements and intended application for your protein. With this information, we use our extensive experience to assist in identifying the optimal expression system for the target protein and its required application.

Our flexible and tailored design stage leverages a number of relevant expression platforms, including mammalian, baculovirus, BacMam, and bacterial (E.coli) cell systems. Test expressions using these platforms can be run in series or in parallel. We offer stable cell line development and banking for future mg to multi-gram productions. We have performed thousands of projects and faced hundreds of different challenges to deliver target proteins.

In addition to our standard Gene to Protein Services, we are pleased to assist in upstream and downstream processing, including Tag addition/removal, endotoxin testing/removal, conjugations, and general optimization of processes.

Timeline for Gene to Protein Services:

  • Construction Design, Gene Synthesis, & Cloning (2-3 weeks)
  • Test Expression & Pilot Purification (2 weeks)
  • Production of mg to Grams w/Low Endotoxin (3-4 weeks)

Deliverables for Gene to Protein Services:

  • QC data for each completed construct & 10 ug of each expression vector
  • Data summary for the test expression, including SDS-PAGE & productivity
  • Purified protein and a complete batch record for production and purification
  • Low endotoxin standard (not e.coli) with extremely low endotoxin option
  • Multiple tag & tag-free options

Complete client satisfaction is our top priority. Kemp Proteins’ Project Managers drive and monitor the operational and technical approach on every project. From initial design through mutually developed milestones, to project delivery and final report, we track and report on:

  • Project timelines
  • Development steps and milestones, including interim deliverables
  • Quality control and tracking of project status and performance through a secured portal
  • Milestone-based communications

Whether you need a small batch of proof-of-principle material using experimental protein, or a larger batch of well-characterized protein, our experienced team understands your protein manufacturing requirements and can alleviate your concerns around putting your production in the hands of another organization. We are committed to delivering quality product to you on time, the first time.

Start your project today! Complete this online form to kick-off discussions:  Online Form for Gene to Protein Services

Gene to Protein Process

Gene to Protein Process

Start your project today! Complete this online form to kick-off discussions:  Online Form for Gene to Protein Services

Mammalian Expression Platform

Production of proteins in appropriate quantity and quality is an essential requirement in today’s pharmaceutical, biotechnology, and diagnostic industries. Expression systems such as CHO (and derivatives) and HEK293 cells are some of the more commonly used for production of various recombinant proteins, especially in large-scale production of therapeutic proteins. Using such mammalian cells increases the level of proper protein folding, post-translational modifications, and product assembly, all vital for complete biological activity. Thus, such cell lines are commonly used for production of antibodies and therapeutic proteins, as well as for proteins that will be used for preclinical animal studies, human therapeutics, and in functional cell-based assays. Kemp has been producing multi-liter/multi-gram protein production in mammalian cell lines for more than 20 years and has a comprehensive compendium of techniques for maximizing productivity, enabling us to provide cost-effective and timely delivery of your specific target.

Mammalian cell lines are often used to provide proteins for functional assays, structural analysis, and protein interactions as well as production of viruses, antibodies, and other complex proteins.

Learn about our mammalian expression services and request a project quote.

Mammalian Cell Line Advantages:
  • Highest-level of protein processing compared to other systems
  • Transient or stable cell line expression
  • Robust optimized transient systems for rapid, ultra high-yield protein production

Production Challenges:
  • Gram-per-liter yields only possible in suspension cultures
  • More demanding culture conditions
  • Expensive production


Baculovirus Expression Platform

Baculovirus expression systems (BEVs) are well established as an easy and reliable way to produce high-quality recombinant proteins approaching that of mammalian cells. These cells are relatively easy to scale up and can be readily adapted to high-density suspension culture for large-scale expression. Expression levels up to 500 mg/L have been reported using the baculovirus expression system. Many post-translational modification pathways present in mammalian systems also occur in insect cells, thus enabling the production of recombinant protein that is similar in antigenicity, immunogenicity, and functionality to native proteins. Kemp has been producing multi-liter/multi-gram protein production using insect cells for more than 20 years and has a comprehensive compendium of techniques for maximizing productivity, enabling us to provide cost-effective and timely delivery of your specific target.

Insect cells are often used to produce proteins for functional assays, structural analysis, protein interactions, and production of viruses.

Learn about our baculovirus expression services and request a project quote.

Baculovirus Cell Line Advantages:
  • Similar to mammalian protein processing
  • Useful in static or suspension culture and less expensive than mammalian cells

Production Challenges:
  • Can be more time-consuming production system
  • More demanding culture conditions than prokaryotic systems


BacMam Expression Platform

The BacMam system uses a modified insect cell virus (baculovirus) as a vehicle to efficiently deliver and transiently express genes in mammalian cells with minimum effort and toxicity. It has a broad range of transduction, including many primary cell types and stem cells; and the level of gene expression can be adjusted by viral dose or chemical additions using histone deacetylase inhibitors. Since the transduction of cells is in liquid phase, BacMam is amenable to automated methods. Baculoviruses are non-replicating in mammalian cells and thus have an excellent safety profile combined with being well-tolerated by cells. The BacMam system has been used to create fluorescent protein-based subcellular markers and biosensors and to introduce genes for pharmacological targets and functional proteins. Kemp has a long history with the BacMam system and was the first company to use the system for industrial-level production. As such, we are your go-to partner for producing proteins via this system.

Learn about our BacMam expression services and request a project quote.

BacMam Advantages:
  • Efficient transduction of mammalian cell lines
  • Portability (assays in pharmacologically relevant cell types)
  • Frozen storage of pre-transduced cells generates assay-ready cells
  • Safety (non-replicating in mammalian cells) and lack of observable cytopathic effect
  • Assay development speed (no need to spend time generating a stable cell line)

Production Challenges:
  • Media can be expensive for large-scale production
  • Large-scale reduction requires production of large volume of virus
  • Glycosylation/folding etc. can be very different to native protein
  • Virus infection can be responsible for host cell death/lysis
  • Potential for protein degradation during expression


Escherichia coli (E.Coli) Expression Platform

Escherichia coli (e. coli) is one of the organisms of choice for the production of recombinant proteins, especially for research and development. Methods and uses as a cell factory are well established. The relatively high yields and simple scalability often make prokaryotic expression systems such as E. coli the most popular expression platform for generating recombinant proteins. Kemp Proteins has decades of experience working with E. coli. Common applications for proteins expressed using E. coli include structural analysis, functional assays, and protein interactions.

Learn about our E. coli expression services and request a project quote.

E. coli Cell Line Advantages:
  • Unparalleled rapid growth time with high-density culture is very scalable
  • Produces high levels of protein expression
  • Simple, inexpensive culture conditions
  • Fast and easy transformation with exogenous DNA

Production Challenges:
  • Potential of inclusion bodies
  • Endotoxin contaminations can limit usefulness
  • Glycosylation/folding etc. can be very different to native protein
  • Some mammalian proteins are difficult to express in E. coli


Final Use of Materials:

  • Target Validation Studies
  • High-Throughput Assay Screening
  • Protein-Protein Interaction Studies
  • In Vivo Toxicology Studies
  • In Vitro Proof of Principle (PoP) Studies
  • Pharmacokinetics (PK) Studies
  • Efficacy Studies
  • OEM for Diagnostic Reagents / Kits
  • Biologics Development
  • Vaccine Development

Gene to Protein Service Quote Request

Once you complete and submit the form, one of our technical experts will will contact you within 1 business day to discuss your project. Thank you.

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