BacMam Expression Services

The industry leader for BacMam mammalian transduction in stirred-tank bioreactors

Kemp Proteins has developed a robust and scalable protein expression platform for BacMam-mediated transductions. We offer a complete line of services, including production, purification, and characterization of recombinant BacMam baculovirus clones as well as process development and scale-up for protein expression.

We optimize BacMam protein expression using titration experiments to determine the optimal virus-to-cell infection ratio and time of harvest.

Cell lysate production, supernatant concentration, and protein purification services are available.

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Not sure which cell line to use? Get in touch.

The BacMam Expression Details:

Multi-host transfection platforms:
(scalable from 1 liter to 100 liters and above)

Mammalian:

CHO (chinese hamster ovary cell line)
HEK-293 (human embryonic kidney cell line)

Our service offerings include:

Gene Synthesis
Virus production
Clone Isolation and Screening
High-Titer Stocks
Virus Banking
Time Course/MOI
Virus Production and Characterization
Bioreactor Volumes from 2-L to 100-L
HEK-293 and CHO-S Host Cell Lines

We express and purify the following proteins:

Monoclonal Antibodies
Viral Glycoproteins
Membrane Proteins
Virus-like Particles (VLPs)
Enzymes

Workflow & Timeline: BacMam Expression

Kemp Proteins BacMam Expression Timeline

Key Publications by Leaders in the BacMam Expression Field:

Kost TA, Kemp CW*. Fundamentals of baculovirus expression and applications. Adv Exp Med Biol. 2016;896:187-97. doi: 10.1007/978-3-319-27216-0_12. Review. PMID: 27165326

O’Grady M, Batchelor RH, Scheyhing K, Kemp CW*, Hanson GT, Lakshmipathy U. BacMam-mediated gene delivery into multipotent mesenchymal stromal cells. Methods Mol Biol. 2011; 698:485-504. doi: 10.1007/978-1-60761-999-4_34. PMID: 21431539

* Kemp Proteins scientist